Cells were stained with PE-anti mouse CD45 (Biolegend, Copenhagen, Denmark) and PerCP-Cy5.5-anti-mouse CD11b (for sorting of whole microglia population) or PerCP-Cy5.5-anti-mouse CD11b, biotinylated-anti-mouse CD11c (BD pharmingen) and PE-anti mouse CD45 (for analysis of CD11c+ microglia) as previously described (8 (link)), and sorted by FACSAriaTM III cell sorter (BD Biosciences, Albortslund Denmark).
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