Trametinib (GSK1120212, Tram) was kindly provided by GlaxoSmithKline (Brentford, Middlesex, UK). Alpelisib (BYL719, Alp), dactolisib (BEZ235, Dact), and Geda (PF05212384) were purchased from Selleck Chemicals (Huston, TX, USA). MK-2206 (MK) was kindly provided by Merck and Co. (Kenilworth, NJ, USA). Alp, Geda, MK, and Tram were dissolved in DMSO as a 1 mM (Geda, MK and Tram) and 5mM (Alp) stock solution, dact was dissolved in DMF as a 1mM. All the drugs were stored at -20°C (Alp, Dact, MK, and Tram) or -80°C (Geda). Eve (RAD001) was kindly provided from Novartis Pharma (Basel, Switzerland) and was dissolved in 100% ethanol as a 10 mM stock solution and stored at -20°C.
The final concentration of drugs was obtained by dilution with culture medium.
Effects on cell growth after 72 h of different treatments were monitored by Crystal Violet assay, as previously described (10 (link)).
The final concentration of drugs was obtained by dilution with culture medium.
Effects on cell growth after 72 h of different treatments were monitored by Crystal Violet assay, as previously described (10 (link)).
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