The cryosection was performed as previously described [35 (link)]. For immunostaining, the primary antibodies were anti-MF20 (DSHB, AB 2147781, 1:50), anti-Glut1 (ABclonal, A6982, 1:200), anti-cmyc (ABclonal, A1309, 1:200), anti-GFP (Abcam, ab13970). The second antibodiess were anti-mouse IgG H&L Alexa Fluor 488 (Abcam, ab150113), anti-Chicken IgY H&L Alexa Fluor 488 (Abcam, ab150169), Dylight 549-conjugated anti-rabbit IgG H&L (EarthOx, E032320), anti-Rabbit IgG H&L Alexa Fluor 647 (Abcam, ab150143), anti-mouse IgG H&L Alexa Fluor 647 (Abcam, ab150115). Nuclei were stained by DAPI (BYT, C1002).
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