The extracts with the use of a pressure vessel were prepared in accordance with the method proposed in the previous study [16 (link)].
Briefly fresh chicory roots after cleaning and grinding (1300 g) were mixed with the solvent in the amount of 2100 mL and extracted with the method proposed by Budryn et al. [17 (link)] with some modifications, in a pressure vessel type PS-5692 (Vienna, Austria). As a solvent, a water–methanol mixture (70/30 or 50/50, v/v) was used at 80 °C for 20 min. The pressure in the vessel during extraction was 0.2 MPa. The obtained suspensions were filtered using the KNF 18 035.3 N vacuum pump (Neuberger, NJ, USA) and paper filters with a density of 84 g/m2 Poch (Gliwice, Poland). The methanol was then evaporated at 446 mbar in a Rotavapor R-210/215 evaporator (Büchi, Switzerland). The obtained extracts, devoid of organic solvent, were frozen at −80 °C for 24 h and freeze-dried (Delta 1-24 LSC lyophilizer, Martin Christ GmbH, Osterode am Harz, Germany). The obtained lyophilized preparations were stored at −25 °C before further analysis.
Briefly fresh chicory roots after cleaning and grinding (1300 g) were mixed with the solvent in the amount of 2100 mL and extracted with the method proposed by Budryn et al. [17 (link)] with some modifications, in a pressure vessel type PS-5692 (Vienna, Austria). As a solvent, a water–methanol mixture (70/30 or 50/50, v/v) was used at 80 °C for 20 min. The pressure in the vessel during extraction was 0.2 MPa. The obtained suspensions were filtered using the KNF 18 035.3 N vacuum pump (Neuberger, NJ, USA) and paper filters with a density of 84 g/m2 Poch (Gliwice, Poland). The methanol was then evaporated at 446 mbar in a Rotavapor R-210/215 evaporator (Büchi, Switzerland). The obtained extracts, devoid of organic solvent, were frozen at −80 °C for 24 h and freeze-dried (Delta 1-24 LSC lyophilizer, Martin Christ GmbH, Osterode am Harz, Germany). The obtained lyophilized preparations were stored at −25 °C before further analysis.
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