The full-length 997bp 3’-UTR of mouse CCN2 was subcloned into a Fire-Ctx sensor lentivector (SBI, Mountain View, CA, USA), downstream of the Firefly luciferase reporter and cytotoxin (CTX) drug sensor genes as described [17 (link)]. Recipient HSC were transfected with parental or CCN2 3’-UTR vectors for 24 hrs prior to 1-hr incubation with RGD, IgG, anti-integrin αvβ3 or anti-integrin α5β1. Cells were then incubated for 24 hrs in the presence of exosomes isolated from Day 1 HSC which we previously showed are highly enriched in miR-214 which directly targets the CCN2 3’-UTR [17 (link)]. To control for transfection efficiency, cells were also transfected with 0.8 μg pRL-CMV vector (Promega, Madison WI, USA) containing Renilla luciferase reporter gene. Luciferase activity was measured in triplicate using an E1910 Dual Luciferase Reporter Assay System (Promega). Renilla luciferase activity was used for normalization, and Firefly luciferase activity in exosome treated cells was compared to that in non-treated cells.