High-Throughput Sequencing Library Preparation

The DNA samples were used with a TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme #TD501) to build the library and VAHTS DNA Clean Beads (Vazyme #N411) for purification. The DNA libraries of 14 samples were sequenced with high-throughput sequencing technology, and each sample contained 30 G of data. The original data obtained by sequencing were converted into sequence data by base calling, which we refer to as raw data or raw reads, and the results were stored in FASTQ (Chen et al., 2018 (link)) format after filtering of low-quality data.

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Zhou T., Ma S., Zhao Y., Guo D., Wang H., Kuang M, & Li X. (2022). Identification and characterization of extrachromosomal circular DNA in alcohol induced osteonecrosis of femoral head. Frontiers in Genetics, 13, 918379.

Publication 2022

TruePrep DNA Library Prep Kit V2 DNA Clean Beads

Dna libraries

Corresponding Organization : Shandong First Medical University

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Variable analysis

independent variables

TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme #TD501) to build the library
VAHTS DNA Clean Beads (Vazyme #N411) for purification

dependent variables

DNA libraries of 14 samples sequenced with high-throughput sequencing technology
Each sample contained 30 G of data

control variables

Kept constant to ensure valid results

controls

No positive or negative controls were explicitly mentioned in the input

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