Live-Dead Cell Identification

The Calcein-AM/Propidium Iodide (PI) dual staining kit (DOjinDO, C542, Japan) was used to label and identify live and dead cells. Calcein-AM removes AM in the presence of esterase within living cells, producing calcein and emitting green fluorescence. PI can be embedded into the DNA double helix of dead cells, emitting red fluorescence. Therefore, green fluorescence represents live cells, and red fluorescence represents dead cells. The operation details were performed according to the instructions.

Free full text: Click here

Cheng P., Zhao X., Han M., Zhuang Y., Ning F., Hu Y., Lu W., Miao S., Zhao C., Jia L., Hao X., Sun M., Wang J., Chen F., Yang L, & Jie Q. (2024). Periodic static compression of micro-strain pattern regulates endochondral bone formation. Frontiers in Bioengineering and Biotechnology, 12, 1356135.

Publication 2024

Corresponding Organization : Xijing Hospital

Other organizations : Ruijin Hospital, Shanghai Jiao Tong University, First Affiliated Hospital of Xi'an Jiaotong University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Live cells (green fluorescence)
Dead cells (red fluorescence)

control variables

None explicitly mentioned

controls

Positive control: Calcein-AM labeling of live cells, emitting green fluorescence
Negative control: Propidium Iodide (PI) labeling of dead cells, emitting red fluorescence

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!