PCa cells (1 × 106), including PC3-vector, PC3-shTNC clones, C4-2b-vector or C4-2b-TNC cells, were injected into the prostate of 6- to 7-week-old male CB.17 scid mice (Taconic Biosciences) [55 (link)]. A total of 10 mice in each group was used, in which 5 mice per cage were randomly allocated as control or experimental group. Control and experimental groups were not blinded to the investigators. Animals were excluded from the analysis when there was no tumor take. Tumor growth was monitored by bioluminescence imaging. DNA was isolated from individual femurs using DNeasy kit. Human-specific Alu qPCR was performed to determine the number of tumor cells in each femur as previously described [11 (link)]. Animal studies were performed in compliance with protocols approved by the Institutional Animal Care and Use Committee at M.D. Anderson Cancer Center.
Protocol detail
Prostate Cancer Tumor Progression Assay
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Corresponding Organization :
Other organizations : The University of Texas MD Anderson Cancer Center, Cold Spring Harbor Laboratory, Baylor College of Medicine
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Variable analysis
independent variables
- Cell lines (PC3-vector, PC3-shTNC clones, C4-2b-vector, C4-2b-TNC cells)
- Tumor growth (monitored by bioluminescence imaging)
- Number of tumor cells in each femur (determined by human-specific Alu qPCR)
- Age of male CB.17 scid mice (6- to 7-week-old)
- Number of mice per group (total of 10 mice, with 5 mice per cage randomly allocated as control or experimental group)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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