16S rRNA Gene Sequencing of Mouse and Human Samples
Corresponding Organization :
Other organizations : University of California, San Francisco, San Francisco Foundation, UCSF Benioff Children's Hospital, Gladstone Institutes, Chan Zuckerberg Initiative (United States)
Variable analysis
- Primer concentration
- DNA template dilution
- Sample indexing
- DNA amplification
- DNA concentration
- Sequencing
- Sequencing read length
- Kapa-HiFi Hotstart (KK2502, Kapa Biosystems) enzyme
- 16S-V4 region primers (V4-515F, V4-806R)
- BioRad CFX 384 real-time PCR instrument
- OpenTrons OT2 for indexing PCR
- Dual GoLay error-correcting index primers
- PicoGreen assay for DNA concentration measurement
- Agencourt AMPure XP magnetic beads for PCR product purification
- Illumina MiSeq and NextSeq 550 platforms for sequencing
- PhiX spike-in for sequencing
- Mouse samples amplified using V4 primers as previously described (38)
- Not explicitly mentioned
Annotations
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