The culture filtrates and mycelia extracts (CEs and MEs) were analyzed by an Agilent (Cernusco sul Naviglio, Milan, Italy) HPLC-MS ESI-TOF 1260/6230DA instrument operating in positive ionization mode. The source temperature was kept at 120 °C and the desolvating gas at 250 °C. The instruments were interfaced to a Phenomenex Luna 5µ C18 column (150 × 4.6 mm, 5 µm). An acetonitrile-water (0.1% formic acid) gradient was used starting from 30% acetonitrile, increasing linearly to 60% in 30 min, 60% for 10 min and finally rebalanced to the initial rate for 5 min. Methanol was used to dissolve the samples. HPLC flow rate was 500 µL min−1.
Pure compounds employed as analytical standards for the qualitative analysis were obtained as previously reported [25 (link),27 (link)].
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