Cell Cycle Analysis of A549 Cells

We performed cell cycle analysis as previously described [18 (link)]. First, we harvested each A549 stably transduced cell group individually. Following PBS washing, the cells were fixed in 3.7% paraformaldehyde and fixed in 70% ethanol. Subsequently, we stained the cells with propidium iodide (MP Biomedicals, LLC, Santa Ana, CA, USA) and ribonuclease-A (Sigma), and analyzed them using a BD FACS Calibur Flow Cytometer, with Cellquest Pro v. 6.0 software (BD Biosciences, Franklin Lakes, NJ, USA). We processed the data we obtained using ModFit LT 5.0 (Verity Software House, Topsham, ME, USA; Supplementary Figure S5). The cell cycle data are presented as the percentage of cell distributions in the different phases (G0/G1, S, and G2).