Generating Liver-Specific SIRT2 Knockout Mice

The liver-specific SIRT2-KO mice were produced by crossing SIRT2^flox/flox mice obtained from Johan Auwerxd Laboratory (Switzerland)^{47 (link)} and Alb-Cre mice purchased from Jackson Laboratory (USA) in a C57BL/6 background. Recombinant AAV8 constructs expressing targeted mouse SIRT2, SIRT2-H187A, LCN2, C/EBPβ, or mutated C/EBPβ (K101R and K102R) under the control of TBG promoter were generated by Sunbio Techservice Inc. (Shanghai, China). A noncoding plasmid carrying only the TBG promoter was used to produce control vector particles. All animals received care in compliance with protocols approved by the Institutional Animal Use and Care Committee of Shanghai Jiaotong University School of Medicine.

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Zhang Y., Long X., Ruan X., Wei Q., Zhang L., Wo L., Huang D., Lin L., Wang D., Xia L., Zhao Q., Liu J., Zhao Q, & He M. (2021). SIRT2-mediated deacetylation and deubiquitination of C/EBPβ prevents ethanol-induced liver injury. Cell Discovery, 7, 93.

Publication 2021

SIRT2flox/flox mice Alb-Cre mice

Animal Care protocols Liver Mouse Plasmid Sirt2

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University, Affiliated Hospital of Youjiang Medical University for Nationalities, Shanghai First People's Hospital

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Variable analysis

independent variables

Liver-specific SIRT2 knockout (SIRT2-KO) vs control (SIRT2-flox/flox)
Recombinant AAV8 constructs expressing targeted mouse SIRT2, SIRT2-H187A, LCN2, C/EBPβ, or mutated C/EBPβ (K101R and K102R)
Control vector particles (noncoding plasmid carrying only the TBG promoter)

dependent variables

Not explicitly mentioned

control variables

C57BL/6 background of mice
TBG promoter used to control expression of genes in AAV8 constructs
Care and use of animals in compliance with protocols approved by the Institutional Animal Use and Care Committee

positive controls

SIRT2-flox/flox mice

negative controls

Control vector particles (noncoding plasmid carrying only the TBG promoter)

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