A total of 156 hexaploid wheat accessions were used to identify Stb16q haplotypes. This collection was composed of 73 SHWs, 45 accessions originated from the wheat core-collection28 (link), 23 recent cultivars, 12 accessions from the wheat Stb differential16 (link), ND495 and two landraces (Supplementary Data 4). Stb16q CDS was entirely sequenced only for accessions carrying the same exon1 as TA4152-19. PCR products were generated using the AmpliTaq Gold® Master Mix (Applied Biosystems), purified using Agencourt AMPure XP (Beckman Coulter), and Sanger sequenced at GATC Biotech SARL (Konstanz, Germany). The MEGA software was used to align the different sequences and to identify haplotypes.
Other organizations :
Génétique, Diversité, Écophysiologie des Céréales, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Wageningen University & Research, Agricultural Research & Education Organization, Université de Bordeaux, Laboratoire de Recherche en Sciences Végétales, Massachusetts Institute of Technology, Cereal Disease Laboratory, Institut Sophia Agrobiotech, ETH Zurich, Centre for Plant Biotechnology and Genomics
Wheat accessions used to identify Stb16q haplotypes
dependent variables
Stb16q haplotypes identified
control variables
SHWs (bread wheat accessions)
Wheat core-collection accessions
Recent cultivars
Wheat Stb differential accessions
ND495
Two landraces
controls
Positive control: TA4152-19 (carrying the same exon1 as the Stb16q CDS)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
