Comprehensive Protein Expression Analysis

Western blotting was performed as described [9 (link)] on 30 μg of protein/sample with antibodies against EZH2 (E7031, Sigma-Aldrich; 1:2000 dilution), actin (sc-1616, Santa Cruz; 1:5000), HA-tag (sc-805, Santa Cruz; 1:2000), histone H3K27me3 (ab6002, Abcam; 1:1000), histone H3K27ac (ab4729, Abcam; 1:1000), total histone H3 (#4499, Cell Signaling; 1:1000), p53 (ab28, Abcam; 1:2000), C/EBP β (sc-7962, Santa Cruz; 1:2000), p300 (#54062, Cell Signaling; 1:500), CBP (#7389, Cell Signaling; 1:500), BRG1 (#49360, Cell Signaling; 1:500), cleaved caspase 3 (#9661, Cell Signaling; 1:1000), and cleaved PARP (#5625, Cell Signaling; 1:1000).

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Zhang H., Zhu D., Zhang Z., Kaluz S., Yu B., Devi N.S., Olson J.J, & Van Meir E.G. (2019). EZH2 targeting in medulloblastoma reduces tumor growth through epigenetic reactivation of the BAI1/p53 tumor suppressor pathway. Oncogene, 39(5), 1041-1048.

Publication 2019

E7031 sc-1616 sc-805 ab6002 ab4729 sc-7962

Actin Antibodies Brg1 Caspase 3 Dilution Ezh2 Histone Histone h3 P300 Protein

Corresponding Organization :

Other organizations : Emory University

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Variable analysis

independent variables

Antibody against EZH2 (E7031, Sigma-Aldrich; 1:2000 dilution)
Antibody against actin (sc-1616, Santa Cruz; 1:5000)
Antibody against HA-tag (sc-805, Santa Cruz; 1:2000)
Antibody against histone H3K27me3 (ab6002, Abcam; 1:1000)
Antibody against histone H3K27ac (ab4729, Abcam; 1:1000)
Antibody against total histone H3 (#4499, Cell Signaling; 1:1000)
Antibody against p53 (ab28, Abcam; 1:2000)
Antibody against C/EBP β (sc-7962, Santa Cruz; 1:2000)
Antibody against p300 (#54062, Cell Signaling; 1:500)
Antibody against CBP (#7389, Cell Signaling; 1:500)
Antibody against BRG1 (#49360, Cell Signaling; 1:500)
Antibody against cleaved caspase 3 (#9661, Cell Signaling; 1:1000)
Antibody against cleaved PARP (#5625, Cell Signaling; 1:1000)

dependent variables

Protein expression levels of EZH2, actin, HA-tag, histone H3K27me3, histone H3K27ac, total histone H3, p53, C/EBP β, p300, CBP, BRG1, cleaved caspase 3, and cleaved PARP

control variables

Amount of protein per sample (30 μg)

Annotations

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