Vascular Perfusion and μCT Imaging

The femoral blood vessels were measured by μCT as previously reported (36 (link)). Briefly, mice were euthanized, the thoracic cavity was opened, a cut was made on the auricula dextra, and a needle was inserted into the left ventricle. Then, the entire vasculature was perfused with 9 ml of warm heparinized saline (100 U/ml in 0.9% saline), 9 ml of 4% paraformaldehyde, another 9 ml of warm heparinized saline, and then 5 ml of Microfil MV-122 (Flow Tech). Next, the femurs were removed and fixed for the detection of μCT.

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Zhang Z., Ding P., Meng Y., Lin T., Zhang Z., Shu H., Ma J., Cohen Stuart M., Gao Y., Wang J, & Zhou X. (2023). Rational polyelectrolyte nanoparticles endow preosteoclast-targeted siRNA transfection for anabolic therapy of osteoporosis. Science Advances, 9(10), eade7379.

Publication 2023

Microfil MV-122

Auricula Blood vessels Femurs Left ventricle Mice Microfil Needle Paraformaldehyde Saline Thoracic cavity

Corresponding Organization : Chinese People's Liberation Army

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Variable analysis

independent variables

Perfusion of the vasculature with different solutions

dependent variables

Measurements of the femoral blood vessels using μCT

control variables

Mouse euthanasia
Opening of the thoracic cavity
Cut made on the auricula dextra
Insertion of a needle into the left ventricle

controls

Positive control: Perfusion of the vasculature with warm heparinized saline (100 U/ml in 0.9% saline)
Negative control: Not explicitly mentioned

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