Modulating Flagellar Genes in E. coli

The ΔflhD deletion allele in strain JW1881-1 (E. coli Genetic Stock Center, Yale Univ.), in which a Km^r gene is substituted for the flhD gene, was transferred to the titratable PtsG strain NQ1243 after deletion of Km^r by phage P1 vir mediated transduction. Similarly, the ΔfliA allele from strain JW1907 (KEIO collection⁴⁵), in which a Km^r gene is substituted for the fliA gene, was transferred to the titratable PtsG strain NQ1243 after deletion of Km^r by phage P1 vir mediated transduction.

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Basan M., Hui S., Okano H., Zhang Z., Shen Y., Williamson J.R, & Hwa T. (2015). Overflow metabolism in E. coli results from efficient proteome allocation. Nature, 528(7580), 99-104.

Publication 2015

Allele Deletion E coli Gene Phage p1 Ptsg Strain

Corresponding Organization :

Other organizations : University of California, San Diego, Scripps Research Institute

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Protocol cited in 15 other protocols

Variable analysis

independent variables

Deletion of the flhD gene
Deletion of the fliA gene

dependent variables

Not explicitly mentioned

control variables

Titratable PtsG strain NQ1243

controls

Positive control: Not specified
Negative control: Not specified

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