Apoptosis Identification in Retinal Cells

Retinal cells undergoing apoptosis were identified by TUNEL assay in retinal cryosections using the DeadEnd Fluorimetric TUNEL system (Promega Corporation, WI) as previously described [24 (link)]. Cells were stained with DAPI to label the nuclei. The images were acquired with a fluorescence microscope (Axio HXP 120, Zeiss, Oberkochen, Germany).

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Alves M.R., Boia R., Campos E.J., Martins J., Nunes S., Madeira M.H., Santiago A.R., Pereira F.C., Reis F., Ambrósio A.F, & Baptista F.I. (2018). Subtle thinning of retinal layers without overt vascular and inflammatory alterations in a rat model of prediabetes. Molecular Vision, 24, 353-366.

Publication 2018

DeadEnd Fluorimetric TUNEL system Axio HXP 120

Apoptosis Assay Cells Cryosections Dapi Fluorescence microscope Fluorimetric Nuclei Promega Retinal Tunel

Corresponding Organization :

Other organizations : University of Coimbra

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Variable analysis

independent variables

Retinal cells undergoing apoptosis

dependent variables

Cells stained with TUNEL assay
Cells stained with DAPI

control variables

Retinal cryosections
DeadEnd Fluorimetric TUNEL system (Promega Corporation, WI)
Fluorescence microscope (Axio HXP 120, Zeiss, Oberkochen, Germany)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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