MTT Cell Viability Assay

After cell treatment, the culture medium was discarded, and the adherent cells were treated for 4 h with 0.5 mg/mL MTT. Then, the MTT was removed, and formazan crystals were dissolved with 100 µL DMSO, including the control group, and quantified by measuring the absorbance at 570 nm in a microplate reader Bio-Rad Xmark spectrophotometer (Bio-Rad Laboratories, Hercules, CA, USA) [28 (link)]. Cell viability was expressed as a percentage of the control. Six experiments were performed in triplicate.

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Gonçalves A.C., Flores-Félix J.D., Costa A.R., Falcão A., Alves G, & Silva L.R. (2021). Hepatoprotective Effects of Sweet Cherry Extracts (cv. Saco). Foods, 10(11), 2623.

Publication 2021

Bio-Rad Xmark spectrophotometer

Cell viability Cells Culture medium Dmso Formazan

Corresponding Organization : Instituto Politécnico da Guarda

Other organizations : University of Coimbra

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Variable analysis

independent variables

Cell treatment

dependent variables

Cell viability (expressed as a percentage of the control)

control variables

Control group

positive controls

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negative controls

Control group

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