Screening K. pneumoniae Colistin Resistance

Within the study collection, 32 out of 50 K. pneumoniae isolates were previously characterized by whole-genome sequencing (WGS), and genetic alterations associated with colistin resistance were formerly investigated (Cannatelli et al., 2014 (link); Di Pilato et al., 2021 (link); Boncompagni et al., 2022 (link)). The remaining colistin resistant isolates (n = 18) were screened for the presence of the most common mcr gene variants by Real-time PCR, including mcr-1 and mcr-2, and additional mcr genes using specific primer/probes combinations (Coppi et al., 2018 (link); Yang et al., 2018 (link)) (Table 1).

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Foglietta G., De Carolis E., Mattana G., Onori M., Agosta M., Niccolai C., Di Pilato V., Rossolini G.M., Sanguinetti M., Perno C.F, & Bernaschi P. (2023). “CORE” a new assay for rapid identification of Klebsiella pneumoniae COlistin REsistant strains by MALDI-TOF MS in positive-ion mode. Frontiers in Microbiology, 14, 1045289.

Publication 2023

Colistin Genes Genetic alterations K pneumoniae Primer Real time pcr

Corresponding Organization : Agostino Gemelli University Polyclinic

Other organizations : Bambino Gesù Children's Hospital, University of Florence, University of Genoa, Azienda Ospedaliero-Universitaria Careggi

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Variable analysis

independent variables

Presence of mcr gene variants

dependent variables

Colistin resistance

control variables

Previously characterized K. pneumoniae isolates by whole-genome sequencing (WGS)

controls

Positive control: mcr gene variants detected by Real-time PCR
Negative control: Not explicitly mentioned

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