MNase assays were performed following the protocol as described previously 3 (link), 39 (link). Briefly, isolated nuclei were digested with 10 U/100 µL MNase in digestion buffer (15 mM Tris-HCl, pH7.4, 15 mM NaCl, 60 mM KCl, 1 mM CaCl2, 0.25 M sucrose, and 0.5 mM DTT) at 37 °C for 10 min. The digested genomic DNA was carefully purified using a DNA purification kit (Axygen, #AP-PCR-250) and subjected to 1.2% agarose gel electrophoresis.
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