DNA-Origami Immobilization and Labeling
Corresponding Organization : Aarhus University
Other organizations : Max Planck Institute of Biochemistry, Ludwig-Maximilians-Universität München, Center for NanoScience
Variable analysis
- Neutravidin concentration (0.1 mg/mL)
- DNA-origami structure concentration (250 pM)
- Gold nanoparticle dilution (1:10)
- MIgG2a-DNA conjugate concentration (60 nM)
- S1-P3 handle concentration (60 nM)
- κLC-Nb-7xR3 concentration (not specified)
- Binding of DNA-origami structures to neutravidin
- Binding of gold nanoparticles to DNA-origami structures
- Binding of mIgG2a-DNA conjugate to DNA-origami structures
- Binding of κLC-Nb-7xR3 to DNA-origami structures
- Flow chamber volume (around 10 µL)
- Flow chamber dimension (1 mm holes at each end)
- Incubation time for neutravidin (5 min)
- Incubation time for DNA-origami structures (15 min)
- Incubation time for gold nanoparticles (5 min)
- Incubation time for mIgG2a-DNA conjugate (15 min)
- Incubation time for S1-P3 handle (15 min)
- Incubation time for κLC-Nb-7xR3 (>45 min)
- Buffer composition (3% BSA in PBS, Buffer B+, 0.5x Buffer C + 1.5% BSA in PBS)
- PH (8.0)
- mIgG2a-DNA conjugate
- S1-P3 handle alone
Annotations
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