Evolutionary Relationships of LHT Genes

To investigate the evolutionary relationships between LHT genes in various plant species, the LHT protein sequences of Arabidopsis thaliana, Oryza sativa, Physcomitrella patens, Selaginella moellendorffii, Medicago truncatula, Solanum lycopersicum and Sorghum bicolor were used to construct an unrooted phylogenetic tree. Multiple-sequence alignment was performed using Clustal X (1.83) software, and the tree was constructed according to the maximum-likelihood (ML) method with the p-distance substitution model in MEGA X software. We used 1000 replicates in a bootstrap analysis to determine a support value for each branch.