Silver ion concentration was determined in different organs after the administration of CS-AgNPs in similar dosing scheduled used for toxicity studies according to the method reported by Lee et al in 2013.37 (link) The animals were sacrificed after 28 days, and tissues were digested in nitric acid using a multiwave microwave digester (Anton Paar, USA). The atomic absorption (AA) spectrophotometer (AA-7000, Shimadzu, Japan) equipped with a graphite furnace (Elmer, USA) was used for the Ag ion determination. The Ag ion concentration in each tissue was calculated in μg/g wet weight. The limit of detection (LOD) and the limit of quantification (LOQ) were found to be 0.29 μg/kg and 0.88 μg/kg, respectively, in the quantification method.
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Silver Ion Quantification in Tissues
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Corresponding Organization :
Other organizations : University of Karachi, Pakistan Council of Scientific & Industrial Research, King Saud University, Prince Sattam Bin Abdulaziz University, Jinnah Sindh Medical University
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Variable analysis
independent variables
- CS-AgNPs administration
- Silver ion concentration in different organs
- Dosing schedule used for toxicity studies
- Tissue digestion in nitric acid using a multiwave microwave digester
- Atomic absorption (AA) spectrophotometer equipped with a graphite furnace for Ag ion determination
- Limit of detection (LOD) and limit of quantification (LOQ) for the quantification method
- No positive or negative controls were explicitly mentioned in the provided information.
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