Enriching Primary Transcripts from Microaerobic Cultures

DNA free total RNA isolated from exponential and microaerobic cultures were treated with TEX (Epicentre #TER51020) to enrich primary transcripts [55 (link)]. For R. sphaeroides RNA Illumina cDNA libraries were prepared by vertis Biotechnology AG, Germany (http://www.vertis-biotech.com/) as described before without prior RNA fragmentation or size fractionation [50 (link)]. Illumina cDNA libraries resulting from TEX treated R. capsulatus RNA were generated as described before without prior rRNA depletion [42 (link)]. cDNA libraries were sequenced on a HiSeq 2000 or HiSeq 2500 machine in single-read mode running 100 cycles. Raw files for R. sphaeroides have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus (GEO) [56 (link)] and are accessible via the GEO accession GSE71844. Raw files for R. capsulatus are accessible via BioProject Accession PRJNA343088.