The expression level of the target protein in chondrocytes of each group was calculated by Western blot and semi-quantitative analysis, using methods we described previously [31 (link)]. The following antibodies were used: TNF-α antibody (1: 1000; Abcam, Cambridge, MA, USA), IL-6 antibody (1: 1000; Abcam, Cambridge, MA, USA), p53 antibody (1: 1000; Cell Signaling Technology, Inc., Danvers, MA, USA), β-actin antibody (1: 2000; Abcam, Cambridge, MA, USA), and secondary antibodies (1: 20 000; Merck Millipore, Darmstadt, Germany).
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Quantification of Protein Expression in Chondrocytes
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Other organizations : Fujian University of Traditional Chinese Medicine
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Variable analysis
independent variables
- Experimental groups
- Expression level of the target protein (TNF-α, IL-6, p53) in chondrocytes
- Western blot and semi-quantitative analysis methods (as described previously [31])
- Antibodies used: TNF-α (1:1000), IL-6 (1:1000), p53 (1:1000), β-actin (1:2000), and secondary antibodies (1:20000)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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