Immunohistochemical Staining of Mouse Cells

Antibody to mouse CD31 (clone 2H8) was purchased from AbDSerotec (Kidlington, UK) and rabbit polyclonal Antibody to NeuN was from Millipore (Darmstadt, Germany). Antibodies to neurofilament 200 and MBP were from Abcam (Cambridge, UK). Monoclonal antibody to β-gal (clone GAL-13) was from Sigma (St. Louis, MO). PE-conjugated anti-mouse CD45 (clone 30-F11), APC-conjugated anti-mouse CD11b (clone M1/70) and PE-conjugated anti-mouse Ly6G (clone 1A8) were from BD Biosciences (Heidelberg, Germany). FITC-conjugated anti-mouse CD4 (clone GK1.5) and PerCP-conjugated anti-mouse CD8 (clone 53-6.7) were from Miltenyi Biotec (Bergisch Gladbach, Germany). APC-conjugated anti-mouse F4/80 (clone BM8), FITC-conjugated anti-mouse IL-17A (clone eBio17B7) and APC-conjugated anti-mouse IFN-γ (clone XMG1.2) and FITC-conjugated anti mouse CD45 were from eBioscience (Frankfurt, Germany). Rat anti-mouse CD8α (clone 53-6.7) was from Novusbio (Littleton, CO). Rabbit polyclonal antibody to IL-17 was from Abcam (Cambridge, UK). Goat anti-rat Alexa 568 and goat anti-rabbit Alexa 488 were from Life Technologies (Darmstadt, Germany). Del-1-Fc was constructed, expressed, and purified as previously described^{17 (link)}.

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Choi E.Y., Lim J.H., Neuwirth A., Economopoulou M., Chatzigeorgiou A., Chung K.J., Bittner S., Lee S.H., Langer H., Samus M., Kim H., Cho G.S., Ziemssen T., Bdeir K., Chavakis E., Koh J.Y., Boon L., Hosur K., Bornstein S.R., Meuth S.G., Hajishengallis G, & Chavakis T. (2014). Developmental endothelial locus-1 (Del-1) is a homeostatic factor in the central nervous system limiting neuroinflammation and demyelination. Molecular psychiatry, 20(7), 880-888.

Publication 2014

Antibody to mouse CD31 (clone 2H8) Antibody to NeuN Antibodies to neurofilament 200 and MBP Monoclonal antibody to β-gal (clone GAL-13) PE-conjugated anti-mouse CD45 (clone 30-F11), APC-conjugated anti-mouse CD11b (clone M1/70) PE-conjugated anti-mouse Ly6G (clone 1A8) FITC-conjugated anti-mouse CD4 (clone GK1.5) PerCP-conjugated anti-mouse CD8 (clone 53-6.7) APC-conjugated anti-mouse F4/80 (clone BM8), FITC-conjugated anti-mouse IL-17A (clone eBio17B7) APC-conjugated anti-mouse IFN-γ (clone XMG1.2) FITC-conjugated anti mouse CD45 Rat anti-mouse CD8α (clone 53-6.7) Goat anti-rat Alexa 568 goat anti-rabbit Alexa 488

Alexa 568 Antibodies Antibody Cd11b Clone Fitc Gal 13 Goat Il 17 Il 17a Monoclonal antibody Mouse Mouse ifn γ Neurofilament 200 Rabbit

Corresponding Organization :

Other organizations : University of Ulsan, Ulsan College, TU Dresden, University of Münster, University of Tübingen, National Institutes of Health, University of Pennsylvania, Goethe University Frankfurt, Polpharma Biologics (Netherlands)

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Antibody to mouse CD31 (clone 2H8) from AbDSerotec
Rabbit polyclonal Antibody to NeuN from Millipore
Antibodies to neurofilament 200 and MBP from Abcam
Monoclonal antibody to β-gal (clone GAL-13) from Sigma
PE-conjugated anti-mouse CD45 (clone 30-F11), APC-conjugated anti-mouse CD11b (clone M1/70) and PE-conjugated anti-mouse Ly6G (clone 1A8) from BD Biosciences
FITC-conjugated anti-mouse CD4 (clone GK1.5) and PerCP-conjugated anti-mouse CD8 (clone 53-6.7) from Miltenyi Biotec
APC-conjugated anti-mouse F4/80 (clone BM8), FITC-conjugated anti-mouse IL-17A (clone eBio17B7) and APC-conjugated anti-mouse IFN-γ (clone XMG1.2) and FITC-conjugated anti mouse CD45 from eBioscience
Rat anti-mouse CD8α (clone 53-6.7) from Novusbio
Rabbit polyclonal antibody to IL-17 from Abcam
Goat anti-rat Alexa 568 and goat anti-rabbit Alexa 488 from Life Technologies
Del-1-Fc constructed, expressed, and purified as previously described

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

None specified

negative controls

None specified

Annotations

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