Human breast cancer cell lines MDA-MB-231, SK-BR-3, and MCF-7 were obtained from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. The LD cell line was established by our laboratory (Department of Pathogenic Biology and Immunology, School of Medicine, Southeast University, Nanjing, China) from a human breast cancer postsurgery sample (8 (link)). All cells were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (GibcoTM; Thermo Fisher Scientific, Inc.). Lipofectamine™ 2000 reagent was obtained from Invitrogen; Thermo Fisher Scientific, Inc. TGF-β1 (Novoprotein Scientific, Inc.) and SB431542 (selective inhibitor of TGF-βRI) (cat. no. A8249; APeXBIO Technology LLC) were used to treat cells.
Protocol detail
Breast Cancer Cell Culture and Manipulation
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Breast cancer cell lines
Cell line
Cells
Chinese
Cultured medium
Fetal bovine serum
Human
Human breast cancer
Lipofectamine 2000
Pathogenic
Sb431542
Tgf β1
Corresponding Organization :
Other organizations : Nanjing Medical University, Jiangsu Province Hospital, Southeast University
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Variable analysis
independent variables
- TGF-β1 treatment
- SB431542 (selective inhibitor of TGF-βRI) treatment
- Not explicitly mentioned
- Cell lines: MDA-MB-231, SK-BR-3, MCF-7, and LD
- Culture medium: Dulbecco's modified Eagle's medium containing 10% fetal bovine serum
- Positive control: TGF-β1 treatment
- Negative control: SB431542 (selective inhibitor of TGF-βRI) treatment
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