Antibodies Used for IHC, IF, and WB

The following primary antibodies were used for immunohistochemistry (IHC), immunofluorescence (IF) and western blots (WB): rabbit anti-Vangl2 (Aviva Systems Biology, San Diego, CA, OAAB15535; 1:200, IHC), rabbit anti-Vangl2 (Ramsbottom et al., 2014 (link)) (21st Century Biochemicals, Marlborough, MA; 1:2000, WB), rabbit anti-Gapdh (Millpore, Temecula, CA, ABS16; 1:30,000, WB), rabbit anti-cofilin (Cell Signaling, Beverly, MA, 5175; 1:1000, WB), rabbit anti-p-cofilin (Ser3) (Cell Signaling, 3313; 1:1000, WB), rabbit anti-β-catenin (Cell Signaling, 9562; 1:1000, WB), mouse anti-β-actin (MP Biomedicals, 08691001; 1:10,000, WB), mouse anti-E-cadherin (BD Biosciences, 610181; 1:2500, WB), rabbit anti-GM130 (Sigma-Aldrich, G7295; 1:1500, IF), rabbit anti-elastin (Biorbyt, orb13391; 1:100, WB), rabbit anti-MMP-12 (Abcam, Ab52897; 1:1000, WB and 1:200, IHC), Armenian hamster anti-Pecam1 (Abcam, Ab119341; 1:500, IF; a gift from Leo Carlin), rabbit anti-phospho-histone H3 (Ser10) (PH3; Millipore, 06-570; 1:1000, IF), rabbit anti-cleaved caspase 3 (D175) (Cell Signaling, 9661; 1:1000, IHC), goat anti-CC10 (T-18) (Santa Cruz Biotechnology, Dallas, TX, sc-9772; 1:1000, IHC), rabbit anti-proSP-C (Millipore, AB3786; 1:1000, IHC), rabbit anti-non-phospho (active) β-catenin (Cell Signaling, 8814; 1:1000, WB).

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Poobalasingam T., Yates L.L., Walker S.A., Pereira M., Gross N.Y., Ali A., Kolatsi-Joannou M., Jarvelin M.R., Pekkanen J., Papakrivopoulou E., Long D.A., Griffiths M., Wagner D., Königshoff M., Hind M., Minelli C., Lloyd C.M, & Dean C.H. (2017). Heterozygous Vangl2Looptail mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair. Disease Models & Mechanisms, 10(4), 409-423.

Publication 2017

rabbit anti-cofilin rabbit anti-β-catenin mouse anti-β-actin mouse anti-E-cadherin rabbit anti-GM130 Ab52897 rabbit anti-cleaved caspase 3 (D175) rabbit anti-proSP-C rabbit anti-non-phospho (active) β-catenin

Actin Antibodies Armenian hamster Cadherin Caspase 3 Cofilin D175 Elastin Gapdh Goat Histone h3 Immunofluorescence Immunohistochemistry Mouse Pecam1 Rabbit Western blots β catenin

Corresponding Organization :

Other organizations : Imperial College London, University College London, Oulu University Hospital, University of Oulu, University of Helsinki, Finnish Institute for Health and Welfare, Royal Brompton & Harefield NHS Foundation Trust, National Institute for Health Research, German Center for Lung Research, Ludwig-Maximilians-Universität München, Mary Lyon Centre at MRC Harwell

Top 5 similar protocols

Variable analysis

independent variables

Vangl2 antibody (Aviva Systems Biology, San Diego, CA, OAAB15535; 1:200, IHC)
Vangl2 antibody (Ramsbottom et al., 2014 (link)) (21st Century Biochemicals, Marlborough, MA; 1:2000, WB)
Gapdh antibody (Millpore, Temecula, CA, ABS16; 1:30,000, WB)
Cofilin antibody (Cell Signaling, Beverly, MA, 5175; 1:1000, WB)
P-cofilin (Ser3) antibody (Cell Signaling, 3313; 1:1000, WB)
β-catenin antibody (Cell Signaling, 9562; 1:1000, WB)
β-actin antibody (MP Biomedicals, 08691001; 1:10,000, WB)
E-cadherin antibody (BD Biosciences, 610181; 1:2500, WB)
GM130 antibody (Sigma-Aldrich, G7295; 1:1500, IF)
Elastin antibody (Biorbyt, orb13391; 1:100, WB)
MMP-12 antibody (Abcam, Ab52897; 1:1000, WB and 1:200, IHC)
Pecam1 antibody (Abcam, Ab119341; 1:500, IF; a gift from Leo Carlin)
Phospho-histone H3 (Ser10) (PH3) antibody (Millipore, 06-570; 1:1000, IF)
Cleaved caspase 3 (D175) antibody (Cell Signaling, 9661; 1:1000, IHC)
CC10 antibody (T-18) (Santa Cruz Biotechnology, Dallas, TX, sc-9772; 1:1000, IHC)
ProSP-C antibody (Millipore, AB3786; 1:1000, IHC)
Non-phospho (active) β-catenin antibody (Cell Signaling, 8814; 1:1000, WB)

dependent variables

Immunohistochemistry (IHC)
Immunofluorescence (IF)
Western blots (WB)

control variables

Vangl2 antibody (Ramsbottom et al., 2014 (link)) (21st Century Biochemicals, Marlborough, MA; 1:2000, WB)
Gapdh antibody (Millpore, Temecula, CA, ABS16; 1:30,000, WB)
β-actin antibody (MP Biomedicals, 08691001; 1:10,000, WB)
Pecam1 antibody (Abcam, Ab119341; 1:500, IF; a gift from Leo Carlin)

Annotations

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