Adriamycin Sensitivity in SGC7901 Cells

The human GC cell line SGC7901 was obtained from the Academy of Military Medical Science (Beijing, China). The Adr -resistant variant of SGC7901 (SGC7901/Adr) was kindly provided by the Fourth Military Medical University (Xi'an, China). Antibodies for SPARC were obtained from Cell Signaling Technology (Beverly, MA, USA), and the secondary goat anti-rabbit and goat anti-mouse antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Adriamycin sensitivity of SGC7901 with or without SPARC siRNA transfection was further investigated using MTT assay. The detailed information and the techniques for cell culture, Western Blot, siRNA, and MTT assay were described in our previous study elsewhere [20 (link), 47 (link)] and the current Supplementary File S1.

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Li Z., Li A.D., Xu L., Bai D.W., Hou K.Z., Zheng H.C., Qu X.J, & Liu Y.P. (2016). SPARC expression in gastric cancer predicts poor prognosis: Results from a clinical cohort, pooled analysis and GSEA assay. Oncotarget, 7(43), 70211-70222.

Publication 2016

secondary goat anti-rabbit and goat anti-mouse antibodies

Adriamycin Anti antibodies Antibodies Assay Cell culture techniques Cell line Goat Human Military Mouse Rabbit Sensitivity Sirna Sparc Transfection Western blot

Corresponding Organization :

Other organizations : China Medical University, First Hospital of China Medical University, Jinzhou Medical University

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Variable analysis

independent variables

SPARC siRNA transfection

dependent variables

Adriamycin sensitivity of SGC7901 cells

control variables

SGC7901 cells without SPARC siRNA transfection

controls

Positive control: SGC7901 cells without SPARC siRNA transfection
Negative control: Not explicitly mentioned

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