AGS, MKN1, NCI-N87 and MGC-803 were treated with demethyltransferase inhibitor (5-Aza, Sigma, St Louis, MO) and histone-deacetylase inhibitor trichostatin A (TSA, Sigma) [23 (link)]. Cells were incubated with 10 μM 5-Aza for 72 h in 5-Aza group, while in TSA group, cells were treated with 100 nM TSA for 24 h. As for the combination treatment group, cells were treated with 5-Aza for 96 h and 100 nM TSA was added into the culture medium in the last 24 h. Equal amount of vehicle DMSO (Sigma) was used in negative control groups.
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