Semi-Intact Drosophila Heart Analysis

Drosophila semi-intact heart preparations were prepared as described previously (Vogler & Ocorr, 2009 (link)). Movies of beating hearts were recorded for 30 s with a high-speed EM-CCD camera (Hamamatsu, C9300) at 130 frames/s. Data were captured using HC Image software (Hamamatsu). Movies were analyzed with Semi-automatic Optical Heartbeat Analysis software to quantify heart periods, systolic and diastolic intervals, systolic and diastolic diameters, fractional shortening, and arrhythmia indexes (defined as the standard deviation of the heart period normalized to the median of each fly) and to produce M-mode records (Cammarato et al., 2015 (link); Fink et al., 2009 (link)).

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Okuda S., Nakamura T., Yamamoto T., Ruoslahti E, & Border W.A. (1991). Dietary protein restriction rapidly reduces transforming growth factor beta 1 expression in experimental glomerulonephritis.. Proceedings of the National Academy of Sciences of the United States of America, 88(21), 9765-9769.

Publication 2023

C9300 HC Image software

Arrhythmia Diastolic Drosophila Frames Heart Heartbeat Systolic

Corresponding Organization : University of South China

Other organizations : Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute, Central South University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Heart periods
Systolic and diastolic intervals
Systolic and diastolic diameters
Fractional shortening
Arrhythmia indexes (standard deviation of the heart period normalized to the median of each fly)

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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