PTZ dose used in this study (40 mg/kg in young mice and 50 mg/kg in adult mice, in 0.9% saline, i.p. injection volume was 1 ml/100 g body weight) was determined as a dose at which PTZ induced seizures in 75% of naive mice. In brief, 2- and 11-wk-old mice treated with drugs (saline, LPS [1.0 mg/kg/d; Sigma-Aldrich], SL327 [selective inhibitor MEK1 and MEK2; 32 mg/kg/d; Tocris Bioscience], or LPS plus SL327, i.p. injection for 3 d [P10–12 or P70–72; see details in the figure legends]) were individually placed in a Plexiglas box, and convulsive behaviors were analyzed for 30 min after PTZ injection. The time to onset and the duration of tonic-clonic seizures were measured as previously described (Shen et al., 2012 (link)).
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PTZ-Induced Seizure Assay in Mice
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Corresponding Organization : First Affiliated Hospital of Wenzhou Medical University
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Variable analysis
independent variables
- Drug treatments: saline, LPS (1.0 mg/kg/d), SL327 (selective inhibitor MEK1 and MEK2; 32 mg/kg/d), or LPS plus SL327
- Age of mice: 2 weeks and 11 weeks
- Time to onset of tonic-clonic seizures
- Duration of tonic-clonic seizures
- PTZ dose: 40 mg/kg in young mice and 50 mg/kg in adult mice, in 0.9% saline
- PTZ injection volume: 1 ml/100 g body weight
- Duration of observation: 30 min after PTZ injection
- Positive control: PTZ administration to naive mice, which induced seizures in 75% of the animals
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