Cell Cycle Regulation Assay

The following chemicals were used: RO3306 (Axon MedChem), Okadaic Acid sodium salt (A.G. Scientifix), Thymidine and 2’-Deoxycytidine hydrate (Santa Cruz Biotechnology). The following antibodies were used; BUB1B (BubR1) (#4116), Cyclin B1 (#12231), Phospho-(Ser) CDK Substrate (#2324), pCdc2-Tyr15 (CDK1-Y15) (#9111), Plk1 (#4535), Aurora B (#3094) and Lamin A/C (#4777) (Cell Signaling Technologies), Securin (ab3306) (Abcam), Cyclin A (sc-596), Cdc2 (CDK1) (sc-137034), Mad2 (sc-47747), Cdc25C (sc-327) (Santa Cruz Biotechnology), centrin (#04–1624) (Millipore) and β-actin (A5441) (Sigma-Aldrich). Anti-Greatwall was obtained as previously described (Vigneron et al., 2009), and monoclonal β-Tubulin hybridoma antibody was a generous donation from Dr Natalie Morin (CRBM, France).

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McCloy R.A., Rogers S., Caldon C.E., Lorca T., Castro A, & Burgess A. (2014). Partial inhibition of Cdk1 in G2 phase overrides the SAC and decouples mitotic events. Cell Cycle, 13(9), 1400-1412.

Publication 2014

RO3306 Thymidine Cyclin B1 Phospho-(Ser) CDK Substrate Aurora B Lamin A/C Cyclin A (sc-596), centrin β-actin

2 deoxycytidine Actin Antibodies Aurora b Axon Bub1b Cdc25c Cdk1 Centrin Crbm Cyclin a Cyclin b1 Hybridoma Lamin a c Monoclonal antibody Morin Okadaic acid Plk1 Ro3306 Salt Securin Sodium Thymidine Tubulin

Corresponding Organization : Faculty (United Kingdom)

Other organizations : Centre de Recherche en Biologie cellulaire de Montpellier, Université de Montpellier

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Protocol cited in 6 other protocols

Variable analysis

independent variables

RO3306 (Axon MedChem)
Okadaic Acid sodium salt (A.G. Scientifix)
Thymidine
2'-Deoxycytidine hydrate (Santa Cruz Biotechnology)

dependent variables

BUB1B (BubR1)
Cyclin B1
Phospho-(Ser) CDK Substrate
PCdc2-Tyr15 (CDK1-Y15)
Aurora B
Lamin A/C
Securin
Cyclin A
Cdc2 (CDK1)
Cdc25C
Centrin
β-actin
Greatwall
β-Tubulin

control variables

None explicitly mentioned

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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