Immunocytochemistry of NS-1 Cells

Upon completion of MS, NS-1 cells were fixed in 4% paraformaldehyde and processed for immunocytochemistry. After blocking in phosphate-buffered saline (PBS) supplemented with 2% Bovine Serum Albumin (BSA) and 0.1% Triton X-100, NS-1 cells were stained with HCS CellMask Red (0.5 μg/ml, Invitrogen, Eugene, OR) (Yeyeodu et al., 2010 (link)) followed by a DAPI-containing mounting medium. Images were captured with the QCapture Pro software and neurite lengths and number were measured using ImageJ.

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Lin C.Y., Huang W.J., Li K., Swanson R., Cheung B., Lin V.W, & Lee Y.S. (2015). Differential Intensity-dependent Effects of Magnetic Stimulation on the Longest Neurites and Shorter Dendrites in Neuroscreen-1 Cells. Journal of neural engineering, 12(2), 026013.

Publication 2015

HCS CellMask Red

Bovine serum albumin Cells Dapi Immunocytochemistry Neurite Paraformaldehyde Phosphate Saline Triton x 100

Corresponding Organization : Cleveland Clinic

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Variable analysis

independent variables

Completion of MS

dependent variables

Neurite lengths
Neurite number

control variables

Fixation in 4% paraformaldehyde
Blocking in phosphate-buffered saline (PBS) supplemented with 2% Bovine Serum Albumin (BSA) and 0.1% Triton X-100
Staining with HCS CellMask Red (0.5 μg/ml)
Mounting with DAPI-containing medium

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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