Preclinical Evaluation of ANRS HIV Vaccine

Animals were immunized twice, two months apart, with the ANRS recombinant MVA-HIV B vaccine (Transgene) at a dose of 4 x 10e⁸ plaque forming units (PFU) by ID injections distributed over 10 injection points (150 μL/point of injections, all along the back, in two columns). Recombinant HIV-1 antigens consisted of the complete sequence of gag, fused to fragments from pol (residues 172-219, 325-383 and 461-519) and nef (residues 66-147 and 182-206) of the Bru/Lai isolate. Blood samples were taken longitudinally before and after immunizations in either lithium-heparin (Greiner Bio-One) for mass cytometry analysis, or in ethylenediaminetetraacetic acid (EDTA) (Greiner Bio-One) for complete blood counts (CBCs) and plasma preparation. The same batch and dose of vaccine, and vaccine schedule, but a different route of administration, were used relative to a previous preclinical study that analyzed innate responses after SC immunization (9 (link)), and for which the mass cytometry dataset was reused here.

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Feraoun Y., Palgen J.L., Joly C., Tchitchek N., Marcos-Lopez E., Dereuddre-Bosquet N., Gallouet A.S., Contreras V., Lévy Y., Martinon F., Le Grand R, & Beignon A.S. (2022). The Route of Vaccine Administration Determines Whether Blood Neutrophils Undergo Long-Term Phenotypic Modifications. Frontiers in Immunology, 12, 784813.

Publication 2021

lithium-heparin

Animals Blood Cbcs Ethylenediaminetetraacetic acid Heparin Hiv antigens Hiv vaccine Immunization Lithium Mva vaccine Plaque Plasma Transgene Vaccine Vaccine schedule

Corresponding Organization :

Other organizations : Infectious Disease Models and Innovative Therapies, Pitié-Salpêtrière Hospital, Sorbonne Université, Inserm, Institut de Recherche Vaccinale

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Variable analysis

independent variables

Route of administration (ID injections vs SC immunization)

dependent variables

Immune responses measured longitudinally before and after immunizations

control variables

Vaccine batch and dose
Vaccine schedule

controls

The same batch and dose of vaccine, and vaccine schedule, but a different route of administration, were used relative to a previous preclinical study that analyzed innate responses after SC immunization.

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