Electrical Coupling in Ant Sensory Dendrites

To investigate the electrical coupling between dendrites of SNs in single basiconic sensilla (Takeichi et al., 2018 (link); Uebi et al., 2022 (link)), we used the general gap junction blocker, carbenoxolone (CBX) (Miriyala et al., 2018 (link)). 3 μL of ant saline (control group) or 10 mM CBX solution (CBX group) was injected into the hemolymph of the ice-anesthetized ant. After incubation for 5–9 h at room temperature, ants were anesthetized again and attached to the acrylic plate for SSRs. In each recording, we extracted waveforms for 2.5 ms before and after the peak of a large amplitude spontaneous spike of a SN and detected all peaks of synchronized spikes within the 5-ms time window. Electrical events were acquired at a sampling rate of 20 kHz, and we defined the spike peak when the waveform potential was increased and decreased for three consecutive sampling points, respectively. In each recording, we counted the number of the large amplitude spontaneous spikes within the 3-s period of recording, and calculated the appearance ratio of the synchronized spikes.

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Watanabe H., Ogata S., Nodomi N., Tateishi K., Nishino H., Matsubara R., Ozaki M, & Yokohari F. (2023). Cuticular hydrocarbon reception by sensory neurons in basiconic sensilla of the Japanese carpenter ant. Frontiers in Cellular Neuroscience, 17, 1084803.

Publication 2023

Ants Carbenoxolone Dendrites Electrical Gap junction Hemolymph Saline Sensilla

Corresponding Organization : Fukuoka University

Other organizations : Hokkaido University, Kobe University, Nara Women's University

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Variable analysis

independent variables

Injection of ant saline (control group) or 10 mM CBX solution (CBX group) into the hemolymph of the ice-anesthetized ant

dependent variables

Number of large amplitude spontaneous spikes within the 3-s period of recording
Appearance ratio of the synchronized spikes

control variables

Incubation time (5–9 h) at room temperature
Sampling rate of electrical events (20 kHz)
Definition of spike peak (waveform potential increased and decreased for three consecutive sampling points)

controls

Positive control: Injection of ant saline (control group)
Negative control: Injection of 10 mM CBX solution (CBX group)

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