After the tumor blocks were fixed in 4% paraformaldehyde overnight, processed and then embedded by paraffin. The tumor tissues were cut into sections of 4 μm thickness, which were mounted on positively charged glass slides by the UArizona Cancer Center TACMASR Core facility for a series of IHC staining processes and procedures following our previous established protocols39 (link). Anti-interferon gamma (ab9657, 1/200), anti-PD-1 (ab137132, 1/500), anti-CD8α (ab209775, 1/100), anti-HMGB1 (ab18256, 1/400), anti-LRP1 (ab92544, 1/750), anti-calreticulin (ab2907, 1/400) and anti-granzyme B (ab4059, 1/100) were purchased from Abcam; anti-PD-L1 (#13684T, 1/75) was purchased from Cell Signaling. All antibodies were diluted in Bond Primary Antibody Diluent (AR9352, Leica). Granzyme B, CD8, HMGB1, and PD-L1 did not require a secondary antibody as they were rabbit antibodies and the HRP-conjugated polymer on the staining kit is against rabbit.