Cultivation of Ganoderma lucidum CGMCC5.26

The strain G. lucidum CGMCC5.26 was purchased from China General Microbiological Culture Collection Center and maintained on potato dextrose agar slants at 4 °C. The seed medium and preculture details of G. lucidum have been described previously [9 (link),11 (link)]. Briefly, the medium was composed of glucose (20 g/L), yeast nitrogen base without amino acids (5 g/L), tryptone (5 g/L), KH₂PO₄ (4.5 g/L), and MgSO₄·7H₂O (2 g/L) at initial pH. The seed was in a 250 mL flask containing 80 mL of medium and was kept at 30 °C on a rotary shaker (150 rpm). In the fermentation medium, the carbon sources were glucose and xylose, and the other components were the same as those in the seed medium. Before inoculating, the seeds were homogenized using an IKA T10 basic homogenizer (IKA, Königswinter, Germany). Briefly, the sterilized dispersing elements of homogenizer inserted the seed medium into a 250 mL flask, and homogenized with the fifth level for 1 min. Then, the homogenized seed medium was centrifuged at 10,000× g for 5 min, and 3.5 g of wet weight cells/L were inoculated into a 500 mL flask with 150 mL of medium. The submerged culture was grown on a rotary shaker at 150 rpm and 30 °C for eight days.