Cultivation and standardization of Candida species

Strains of C. albicans (ATCC 90028) and C. glabrata (ATCC 2001) were cultivated aerobically on Sabouraud Dextrose (Merck KGaA, Germany) agar at 37 °C. Cell suspensions were grown in RPMI 1640 (Inlab diagnóstica, Brazil) during 24 h at 37 °C. Before experiments, cells were centrifuged (5000 g for 5 min), washed twice with sterile saline, and suspended in RPMI 1640 medium. Suspensions were standardized at OD₆₀₀ = 1.0 (1 × 10⁶ cells/mL) (LGL Scientific 0741/16, Brazil), based in experiments described previously with some adaptations [22 (link)].

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Martorano-Fernandes L., Cavalcanti Y.W, & de Almeida L.D. (2020). “Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”. BMC Complementary Medicine and Therapies, 20, 104.

Publication 2020

Sabouraud Dextrose

Adaptations Agar C glabrata Cells Dextrose Saline Sterile Strains

Corresponding Organization :

Other organizations : Universidade Federal da Paraíba

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Variable analysis

independent variables

Strains of C. albicans (ATCC 90028) and C. glabrata (ATCC 2001)

dependent variables

Not explicitly mentioned

control variables

Cultivation of strains on Sabouraud Dextrose (Merck KGaA, Germany) agar at 37 °C
Cell suspensions grown in RPMI 1640 (Inlab diagnóstica, Brazil) during 24 h at 37 °C
Cells centrifuged (5000 g for 5 min), washed twice with sterile saline, and suspended in RPMI 1640 medium
Suspensions standardized at OD600 = 1.0 (1 × 10^6 cells/mL) (LGL Scientific 0741/16, Brazil)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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