Quantification of Shiga Toxin 2
Corresponding Organization :
Other organizations : Kurume University, Kyushu University, Miyazaki Prefectural Hospital, Osaka Prefectural Institute of Public Health
Variable analysis
- Addition of mitomycin C (MMC) to the bacterial cell culture
- Stx2 concentration in the cell lysates
- Temperature (37 °C)
- Shaking of the bacterial cell culture
- Bacterial growth to mid-log phase
- Volume of the bacterial cell culture (40 ml)
- Centrifugation conditions (14,000 × g, 4 °C, 10 min)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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