Olink Explore 1536 COVID-19 Protein Profiling

Detailed description is available online (https://www.olink.com/mgh-covid-study/) (Fig 1) and has been published previously [29 (link)]. Briefly, the samples were analyzed by the Olink® Explore 1536 platform which includes measurement of the ACE2 protein. The Olink platform is based on Proximity Extension Assay (PEA) technology and has been validated previously [31 (link)]. Data generation consists of three main steps: normalization to known standard (extension control), log2-transformation, and level adjustment using the plate control. The generated data represent relative protein values, Normalized Protein eXpression (NPX), on a log2 scale where a larger number represents a higher protein level in the sample. For more information about Olink® Explore 1536, PEA and NPX, please visit www.olink.com.

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Kragstrup T.W., Singh H.S., Grundberg I., Nielsen A.L., Rivellese F., Mehta A., Goldberg M.B., Filbin M.R., Qvist P, & Bibby B.M. (2021). Plasma ACE2 predicts outcome of COVID-19 in hospitalized patients. PLoS ONE, 16(6), e0252799.

Publication 2021

Olink® Explore 1536

A protein Ace2 protein Assay Protein

Corresponding Organization : Lundbeck Foundation

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein levels of ACE2 measured using the Olink® Explore 1536 platform

control variables

Normalization to known standard (extension control)
Log2-transformation
Level adjustment using the plate control

controls

Positive control: Known standard for normalization
Negative control: Plate control for level adjustment

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