Parathyroid Hormone Peptide Ligand Assay

The peptide ligands parathyroid hormone PTH (1 (link)–34 (link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, no link found, no link found, link, link, link, link, link)) (human, H-4835-GMP, 4033364) and parathyroid hormone-related peptide (PTHrP) (1 (link)–34 (link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, no link found, no link found, link, link, link, link, link)) (human, mouse, rat; 4017147) were from Bachem. SNAP-Cell 647-SiR (S9102S) was from New England Biosciences. Anti–HA-tag antibody (ab9110) was from Abcam and anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (7074P2) was from Cell Signaling. The 3,3′,5,5′-tetramethylbenzidine (TMB, T8665) was from Sigma-Aldrich. NanoBRET Nano-Glo Substrate–furimazine (N1663) and HaloTag NanoBRET 618 Ligand (G9801) were from Promega. Dimethyl sulfoxide (A994.2) for cell culture was from Carl Roth GmbH & Co. KG. Bovine serum albumin (BSA, SAFSA7030) was from VWR International.

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Nemec K., Schihada H., Kleinau G., Zabel U., Grushevskyi E.O., Scheerer P., Lohse M.J, & Maiellaro I. (2022). Functional modulation of PTH1R activation and signaling by RAMP2. Proceedings of the National Academy of Sciences of the United States of America, 119(32), e2122037119.

Publication 2022

H-4835-GMP, Anti–HA-tag antibody (ab9110)

Anti antibody Anti igg Antibody Bovine serum albumin Cell Cell culture Dimethyl sulfoxide Furimazine Halotag Horseradish peroxidase Human Ligand Mouse Parathyroid hormone Peptide Promega Pthrp Rabbit Tetramethylbenzidine

Corresponding Organization :

Other organizations : University of Würzburg, Max Delbrück Center, Karolinska Institutet, Charité - Universitätsmedizin Berlin, German Centre for Cardiovascular Research, Queen's Medical Centre, University of Nottingham

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Variable analysis

independent variables

Peptide ligands parathyroid hormone PTH (1-34) (human, H-4835-GMP, 4033364) and parathyroid hormone-related peptide (PTHrP) (1-34) (human, mouse, rat; 4017147)

dependent variables

Not explicitly mentioned

control variables

SNAP-Cell 647-SiR (S9102S) from New England Biosciences
Anti–HA-tag antibody (ab9110) from Abcam
Anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (7074P2) from Cell Signaling
3,3′,5,5′-tetramethylbenzidine (TMB, T8665) from Sigma-Aldrich
NanoBRET Nano-Glo Substrate–furimazine (N1663) and HaloTag NanoBRET 618 Ligand (G9801) from Promega
Dimethyl sulfoxide (A994.2) for cell culture from Carl Roth GmbH & Co. KG
Bovine serum albumin (BSA, SAFSA7030) from VWR International

positive controls

Not specified

negative controls

Not specified

Annotations

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