Glucose Quantification and Signaling in Cacao

CLPr was prepared from cacao liquor and its composition was previously described.^{(17 (link),19 (link))} Glucose was measured using a commercially available kit [Labassay Glucose Wako kit (FUJIFILM Wako Pure Chemical Co., Ltd., Osaka, Japan)]. Antibodies against β-actin, AMPKα, phospho-AMPKα (thr172), phospho-CaMKK2 (ser511), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), liver kinase B1 (LKB1) and phospho-LKB1 (ser428) were purchased from Cell Signaling Technology Co. (Denver, MA). Antibodies against CaMKK2 and GLUT4 were purchased from Abcam (Hercules, CA). Horseradish peroxidase (HRP)-conjugated anti-mouse IgG, HRP-conjugated anti-goat IgG antibodies, anti-insulin receptor (IR), and anti-Lamin B were from Santa Cruz Biotechnology (Santa Cruz, CA). HRP-conjugated anti-rabbit IgG was from Bio-Rad Laboratories Inc. (Hercules, CA). All other reagents used were of the highest grade available from commercial sources.

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Hironao K.Y., Ashida H, & Yamashita Y. (2020). The cacao procyanidin extract-caused anti-hyperglycemic effect was changed by the administration timings. Journal of Clinical Biochemistry and Nutrition, 67(1), 61-66.

Publication 2020

β-actin Horseradish peroxidase (HRP)-conjugated anti-mouse IgG anti-Lamin B HRP-conjugated anti-rabbit IgG

Actin Anti igg Antibodies Cacao Glucose Glut4 Glyceraldehyde 3 phosphate dehydrogenase Goat Horseradish peroxidase Insulin receptor Lamin b Liquor Liver kinase b1 Mouse Rabbit

Corresponding Organization :

Other organizations : Kobe University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Glucose levels

control variables

β-actin
AMPKα
Phospho-AMPKα (thr172)
Phospho-CaMKK2 (ser511)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Liver kinase B1 (LKB1)
Phospho-LKB1 (ser428)
CaMKK2
GLUT4
Insulin receptor (IR)
Lamin B

controls

Positive control: Not specified
Negative control: Not specified

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