Total leukocytes (CD45+CD41− cells; fig. S8A) were analyzed using Cytobank software and equal cell numbers were sampled for unsupervised, high-dimensional visualization t-Stochastic Neighbor Embedding (viSNE) analysis (33 (link)). Thirteen parameter viSNE maps were generated from antibodies against the following cell surface markers: CD8, CD4, CD20, CD11c, CD11b, CD123, CD13, CD56, CD33, CD15, CD3, CD14, and HLA-DR. For Spanning-tree Progression Analysis of Density-normalized Events (SPADE) analysis (55 (link)), mononuclear cells (CD45+CD41−CD15− cells; fig. S8A) were analyzed with Cytobank software (Cytobank, Inc.). The down-sampled events were then clustered based on phenotypically similar cells by the expression of CD4, CD8, CD3, CD14, CD16, CD56, CD20, CD123, CD33, CD13, HLADR, CD11c, and CD11b. Because the largest single-cell increases in the abundances of intracellular phosphoproteins occurred at 15 min compared to initial experiments performed at 1 and 5 min after incubation with each SPM (fig. S7), 15-min incubations were thus used throughout. To determine the fold-difference in the abundances of phosphoproteins of interest, vehicle controls were used as reference points and compared to samples incubated with SPMs.