Cellular suspensions were loaded onto the 10x Chromium instrument (10x Genomics) and sequenced as described in Zheng et al17 (link). The cDNA libraries were sequenced using a custom program on 10 lanes of Illumina HiSeq2500 Rapid Mode, yielding 1.8B total reads and 25K reads per cell. At these depths, we recovered >90% of captured transcripts in each sequencing experiment.
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Corresponding Organization : University of Michigan–Ann Arbor
Other organizations : University of California, San Francisco, University of British Columbia
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Variable analysis
independent variables
- Cellular suspensions
- Sequencing reads
- Recovered transcripts
- Sequencing platform (10x Chromium instrument)
- Sequencing method (Illumina HiSeq2500 Rapid Mode)
- Sequencing depth (1.8B total reads, 25K reads per cell)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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