Immunostaining of Mouse Embryonic Stem Cells

Mouse ESCs were cultured in 8-well slides (Ibidi). ESC immunostaining was carried out as previously described in Canham et al., 2010 (link). The following antibodies were used: anti-NANOG (eBioscience, 14-5761, 1:200), anti-OCT4 (Santa Cruz, sc-5279, 1:200), and anti-GATA6 (Cell Signalling Technologies, 5851, 1:1600). Secondary antibodies used are from the Alexa Fluor series (Molecular Probes, Thermo Fisher). mESCs were imaged using a Deltavision Widefield Screening microscope.

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Perera M., Nissen S.B., Proks M., Pozzi S., Monteiro R.S., Trusina A, & Brickman J.M. (2022). Transcriptional heterogeneity and cell cycle regulation as central determinants of Primitive Endoderm priming. eLife, 11, e78967.

Publication 2022

anti-NANOG anti-OCT4 anti-GATA6 Alexa Fluor series

Antibodies Escs Mescs Microscope Molecular probes Mouse Oct4

Corresponding Organization :

Other organizations : Novo Nordisk Foundation, University of Copenhagen

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Variable analysis

independent variables

Antibody type (anti-NANOG, anti-OCT4, anti-GATA6)

dependent variables

Immunostaining of mouse embryonic stem cells (mESCs)

control variables

Cell culture conditions (8-well slides, Ibidi)
Immunostaining protocol (as previously described in Canham et al., 2010)
Microscopy technique (Deltavision Widefield Screening microscope)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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