The LC-MS/MS analysis was carried out according to the procedure described previously [17 (link)]. Peptides were dissolved in 0.1% formic acid, directly loaded onto a reversed-phase pre-treated Acclaim PepMap 100 column (Thermo scientific, Shanghai, China). Peptide separation was carried out using a reversed-phase analytical Acclaim PepMap RSLC column (Thermo scientific, Shanghai, China). The resulting peptides were analyzed by Q Exactive™ plus hybrid quadrupole-Orbitrap MS (Thermo scientific, Shanghai, China).
The peptides were subjected to NSI source followed MS/MS in Q Exactive™ plus coupled online to the UPLC system (Thermo scientific, Shanghai, China). Intact peptides were detected in the Orbitrap at a high resolution of 70,000 and ion fragments were detected in the Orbitrap at a low resolution of 17,500. For MS scans, the m/z scan range was 350 to 1800, and the first mass was set as 100 m/z. The mass spectrometry proteomics data have already been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009584 (http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD009584 ).
The peptides were subjected to NSI source followed MS/MS in Q Exactive™ plus coupled online to the UPLC system (Thermo scientific, Shanghai, China). Intact peptides were detected in the Orbitrap at a high resolution of 70,000 and ion fragments were detected in the Orbitrap at a low resolution of 17,500. For MS scans, the m/z scan range was 350 to 1800, and the first mass was set as 100 m/z. The mass spectrometry proteomics data have already been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009584 (
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