Betaine anhydrous (N, N, N-trimethylglycine) was acquired from Sigma-Aldrich (St. Louis, MO, USA) and diluted in 0.9% normal saline for IV infusion. Urethane was purchased from Sigma-Aldrich (Poznań, Poland).
For high-performance liquid chromatography-mass spectrometry (HPLC-MS) betaine hydrochloride, tert-butyl bromoacetate (TBBA), ammonia solution and ammonium formate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Betaine-D3 hydrochloride was obtained from Toronto Chemicals Research (North York, Canada). The stock solution of betaine was prepared fresh in methanol. LC-MS grade acetonitrile, HPLC grade acetone, HPLC grade methanol and formic acid were obtained from J.T. Baker (Phillipsburg, New Jersey, USA). Ultra-pure water (Mili-Q water) was produced by a water purification system (Mili-Q, Millipore, Milford, MA, USA).
For high-performance liquid chromatography-mass spectrometry (HPLC-MS) betaine hydrochloride, tert-butyl bromoacetate (TBBA), ammonia solution and ammonium formate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Betaine-D3 hydrochloride was obtained from Toronto Chemicals Research (North York, Canada). The stock solution of betaine was prepared fresh in methanol. LC-MS grade acetonitrile, HPLC grade acetone, HPLC grade methanol and formic acid were obtained from J.T. Baker (Phillipsburg, New Jersey, USA). Ultra-pure water (Mili-Q water) was produced by a water purification system (Mili-Q, Millipore, Milford, MA, USA).
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