Spectroscopic Analysis of Natural Compounds

Similar to previous procedures [10 (link),15 (link),16 (link),17 (link),23 (link)], Autopol VI polarimeters were applied to determine optical rotations. The Chirascan CD spectrometer was applied to measure UV and ECD data. The Nicolet iS50 FT-IR spectrometer was applied to acquire IR data. The Bruker Avance III 500 NMR spectrometer (500 MHz for ¹H and 125 MHz for ¹³C) was applied to record 1D/2D NMR data. The Xevo G2-XS QTof mass spectrometer was applied to obtain HRESIMS data. The Agilent 1260 Infinity II system with a ZORBAX SB-C18 (5 μm, 9.4 × 250 mm) column was applied for HPLC separation. Silica gel (200–300 mesh, Qingdao Haiyang Chemical Co., Qingdao, China), RP-18 (AAG12S50, YMC Co., Ltd., Kyoto, Japan), and Sephadex LH-20 (GE Healthcare, Chicago, IL, USA) were employed for column chromatography (CC). Precoated silica gel plates (GF-254, 20 × 20 cm, Qingdao Haiyang Chemical Co., Qingdao, China) were used for thin-layer chromatography (TLC).

Free full text: Click here

Li C.P., Shi Z.Z., Fang S.T., Song Y.P, & Ji N.Y. (2023). Lipids and Terpenoids from the Deep-Sea Fungus Trichoderma lixii R22 and Their Antagonism against Two Wheat Pathogens. Molecules, 28(17), 6220.

Publication 2023

Chirascan CD spectrometer Avance III 500 NMR spectrometer SB-C18 RP-18 (AAG12S50, Sephadex LH-20 GF-254

Chromatography Hplc Optical rotations Sephadex lh 20 Silica gel Thin layer chromatography

Corresponding Organization : Research Center of Saline and Akali Land of State Foresty Administration

Top 5 similar protocols

Variable analysis

independent variables

Application of Autopol VI polarimeters to determine optical rotations
Application of Chirascan CD spectrometer to measure UV and ECD data
Application of Nicolet iS50 FT-IR spectrometer to acquire IR data
Application of Bruker Avance III 500 NMR spectrometer (500 MHz for 1H and 125 MHz for 13C) to record 1D/2D NMR data
Application of Xevo G2-XS QTof mass spectrometer to obtain HRESIMS data
Application of Agilent 1260 Infinity II system with a ZORBAX SB-C18 (5 μm, 9.4 × 250 mm) column for HPLC separation
Use of silica gel (200–300 mesh, Qingdao Haiyang Chemical Co., Qingdao, China), RP-18 (AAG12S50, YMC Co., Ltd., Kyoto, Japan), and Sephadex LH-20 (GE Healthcare, Chicago, IL, USA) for column chromatography (CC)
Use of precoated silica gel plates (GF-254, 20 × 20 cm, Qingdao Haiyang Chemical Co., Qingdao, China) for thin-layer chromatography (TLC)

dependent variables

Optical rotations
UV and ECD data
IR data
1D/2D NMR data
HRESIMS data
HPLC separation

control variables

Autopol VI polarimeters
Chirascan CD spectrometer
Nicolet iS50 FT-IR spectrometer
Bruker Avance III 500 NMR spectrometer
Xevo G2-XS QTof mass spectrometer
Agilent 1260 Infinity II system with a ZORBAX SB-C18 (5 μm, 9.4 × 250 mm) column
Silica gel (200–300 mesh, Qingdao Haiyang Chemical Co., Qingdao, China), RP-18 (AAG12S50, YMC Co., Ltd., Kyoto, Japan), and Sephadex LH-20 (GE Healthcare, Chicago, IL, USA) for column chromatography (CC)
Precoated silica gel plates (GF-254, 20 × 20 cm, Qingdao Haiyang Chemical Co., Qingdao, China) for thin-layer chromatography (TLC)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!