All
chemical reagents were used without further
purification, unless otherwise specified. 4-Cyano-4-(phenylcarbonothioylthio)pentanoic
acid N-succinimidyl ester (RAFT-NHS), (3-aminopropyl)triethoxysilane
(APTES), TEOA, EY, triethylamine (TEA), oligo(ethylene glycol) methyl
ether methacrylate (MeOEGMA, average Mn 300), ethanol (EtOH, 99.9%), acetone (99.5%), dry tetrahydrofuran
(THF, 99.9%), and phosphate-buffered saline (PBS) were purchased from
Sigma-Aldrich; HPMA was obtained from Polysciences, Inc.; and streptavidin-Alexa488
conjugate (Str-Alexa488) and BS albumin-Alexa488 conjugate (BSA-Alexa488)
were purchased from Fisher Thermo Scientific. Silicon substrates were
acquired from Siltronix. Deionized water was produced using a Milli-Q
integral 3 system (Millipore, Molscheim, France). (3-Acryloylamino-propyl)-(2-carboxy-ethyl)-dimethyl-ammonium
(CBMA) was synthesized according to a previously described procedure.11 (link),12 (link),20 (link) BS was obtained and biotinylated
as previously described.21 (link)
chemical reagents were used without further
purification, unless otherwise specified. 4-Cyano-4-(phenylcarbonothioylthio)pentanoic
acid N-succinimidyl ester (RAFT-NHS), (3-aminopropyl)triethoxysilane
(APTES), TEOA, EY, triethylamine (TEA), oligo(ethylene glycol) methyl
ether methacrylate (MeOEGMA, average Mn 300), ethanol (EtOH, 99.9%), acetone (99.5%), dry tetrahydrofuran
(THF, 99.9%), and phosphate-buffered saline (PBS) were purchased from
Sigma-Aldrich; HPMA was obtained from Polysciences, Inc.; and streptavidin-Alexa488
conjugate (Str-Alexa488) and BS albumin-Alexa488 conjugate (BSA-Alexa488)
were purchased from Fisher Thermo Scientific. Silicon substrates were
acquired from Siltronix. Deionized water was produced using a Milli-Q
integral 3 system (Millipore, Molscheim, France). (3-Acryloylamino-propyl)-(2-carboxy-ethyl)-dimethyl-ammonium
(CBMA) was synthesized according to a previously described procedure.11 (link),12 (link),20 (link) BS was obtained and biotinylated
as previously described.21 (link)
